Natto NKCP Stability

Stability data on natto NKCP

1. STORAGE STABILITY

The dried filtrate of B. subtilis natto culture (NKCP Nattokinase) was examined for changes in the content of natto bacillus-produced proteins and peptidase activity during storage. No changes in the content and activity were observed after 6 months of storage at 37ºC or 24 months of storage at room temperature.

Stability of NKCP peptidase activity

Changes in peptidase activity of NKCP powder were ±5% or less when stored at 25ºC or 37ºC for 6 months.

2. HEAT STABILITY

A solution of 1g of NKCP powder in 10ml of 50mM tris–hydrochloric acid buffer (containing 100mM of NaCl, pH 9.0) was used in a heat-stability test for peptidase activity. The NKCP solution, treated under different temperatures and times, was examined for the residual rate of peptidase activity of B. subtilis natto produced protein using a synthetic chromatogenic substrate, S-2251. As shown in the figure below, the peptidase in NKCP was stable when heated up to 60ºC.

Heat-stability of peptidase activity

We use cookies to improve your experience on our website. For more details see our cookie policy.

Like many other websites, we use cookies to provide enhanced functionality and track the usage of our websites with Google Analytics, and some of our third-party advertisers might use them to deliver adverts tailored to what they think you might be interested in.

What are cookies?

Cookies are small text files which can be sent to browser when you visit websites and saved to your device (computer or other device with Internet access, i.e. smartphone or tablet). Cookies are saved in the folder for files of your browser. Cookies usually contain the name of the website they come from and the date of origin. Upon your next visit the browser loads cookies again and sends these information back to the website which originally created cookies. Cookies we use do not harm your computer.

Using cookies

By using this website you give your consent to using cookies in compliance with your browser settings. If you visit our websites, acceptance of cookies is allowed in your browser, you do not change your browser settings and continue visiting our websites we consider it as accepting our conditions for using cookies.

Why are we using cookies?

Cookies are used to optimally create and constantly improve the quality of our services, adjust them to your interests and needs and improve their structure and contents. We do not use data obtained by using cookies to contact users by mail, e-mail or telephone.

How to change settings of cookies?

Majority of browsers have been originally set to automatically accept cookies. This settings can be changed by blocking cookies or by a notification in case cookies are to be sent to your device. Instructions for changing cookies are available in the option “Help” of every browser. If you use different devices to access websites (e.g. computer, smartphone, tablet), we recommend you to adjust every browser on each of them to your preferences regarding cookies.

Why to keep settings of cookies?

Using cookies and permitting cookies in your browser is your decision. However, in case you change settings of cookies, functionality of some of our websites can be limited and their user comfort reduced.